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Representative radio-HPLC chromatograms of 1 prepared from free hydrazine (left) and hydrazone (right). The macrocyclic 99mTc complex prepared from free-hydrazine or hydrazone-protected K(HYNIC)2-RGD2 had identical radio-HPLC profiles under the same chromatographic conditions, suggesting that both hydrazone-protecting groups in K(HYNIC)2-RGD2 were removed during 99mTc chelation.
