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. 2013 Apr 16;2(5):362–375. doi: 10.5966/sctm.2012-0135

Figure 2.

Figure 2.

In vitro biological assays show functional hsTNFR activity in hsTNFR-transduced hMSCs. (A): hsTNFR-transduced hMSC conditioned medium inhibits hTNFα-mediated nitric oxide release from bovine articular chondrocytes. Griess analysis was done of bovine articular chondrocytes incubated with human TNFα (10 ng/ml) or IL-1α (10 ng/ml) plus conditioned supernatant from hsTNFR-transduced hMSCs (four different donors). Error bars indicate standard deviation (n = 4). (B): Expression of hsTNFR prevents human TNFα induction of ICAM-1. The indicated amounts of TNFα were added to untransduced or hsTNFR-transduced hMSCs. The cells were harvested 3 days post-treatment, and the percentage of ICAM-1-positive cells was analyzed using fluorescence-activated cell sorting. Analysis was done on a single culture (one well of a six-well panel). Similar results were obtained from hMSC donors 225 and 310. Abbreviations: hMSC, human mesenchymal stem cell; hsTNFR, human soluble tumor necrosis factor receptor II; ICAM, anti-intercellular adhesion molecule; IL, interleukin; NO, nitric oxide; TNFα, tumor necrosis factor-α.