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. 2013 Apr 18;2(5):384–393. doi: 10.5966/sctm.2012-0163

Figure 3.

Figure 3.

Differentiating cells rapidly acquire early eye field and retinal pigmented epithelium (RPE) marker expression. (A): Immunofluorescence images of pluripotency (Oct4), early eye field (Pax6, Lhx2, and Otx2), and RPE (Pax6, Lhx2, Mitf, Otx2, and Pmel17) proteins during days 0–14 of differentiation. Scale bars = 100 μm. (B): Quantitative polymerase chain reaction analysis of pluripotency (Oct4 and Nanog), early eye field (Lhx2, Pax6(−5a), Pax6(+5a), Rax, and Otx2), and RPE (Lhx2, Pax6(−5a), Pax6(+5a), Otx2, Mitf, Pmel17, PEDF, BEST1, Tyrosinase, and Tyrp2) gene expression. Expression is normalized to the maximum level of expression over the time period. The error bars represent the standard error of the mean.