Fig. 4.

Isolation ofE. helvumadenovirus 1 (AdV1). (A) Pteropus alecto primary kidney cells showing cytopathic effect (and control) at day 6 post infection with E. helvum AdV1. Magnification is 200 X (B) Transmission electron micrograph of a Pteropus alecto cell infected with E. helvum AdV1. Arrow indicates adenovirus particles observed in the nucleus (N). Bar represents 200 nm. Insert adenovirus particle negative stained from supernatant of the same culture. Insert bar represents 100 nm. (C) Midpoint-rooted phylogenetic tree based on alignment of hexon proteins (915aa) of E. helvum AdV1 (starred) and representative adenoviruses. Genera are demarcated by adjacent lines and names. Posterior probability values are shown and the bar represents the expected number of amino acid substitutions per site.