Figure 10. The effector gene Avr3a and its cognate resistance gene R3a.
(A) Diagram of AVR3A effector protein. (B) Frequency of Avr3a alleles in historic and modern P. infestans strains. (C) Neighbor-joining tree of R3a homologs from potato, based on 0.67 kb partial nucleotide sequences of S. tuberosum R3a (blue, accession number AY849382.1) and homologs (dark grey) in GenBank, and de novo assembled contigs from M-0182896 (red). Numbers at branches indicate bootstrap support with 500 replicates. Scale indicates changes.
Figure 10—figure supplement 1. Summary of de novo assembly of RXLR effector genes.
TBLASTN query was performed with 549 RXLR proteins as a query and contigs as a database. When the High-scoring Segment Pair (HSP) and matched amino acids both covered ≥99% of the query length, we recorded a hit. Results with the optimal k-mer size are highlighted.