Figure 3.
Comparison of transient averaging and multiple fills for selected product ions of Histone H3.3. The average S/N of 10 representative product ions from Fig. 4 as a function of the number of microscans (A-QLT ETD) or fill (MDC ETD). B) The average elapsed MS/MS scan time to perform A-QLT ETD using microscans or MDC ETD using multiple fills. These values are from histone infusion experiments where the average time required to accumulate 5×105 precursor charges for MS/MS was ~ 4.5 ms. The Orbitrap resolving power was set to 60k (768 ms transient).