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. Author manuscript; available in PMC: 2013 May 30.
Published in final edited form as: Immunogenetics. 2009 Nov 24;62(1):41–48. doi: 10.1007/s00251-009-0404-9

FIGURE 3. Genetic control of LPS response in different B cell subpopulations from spleen and peritoneal cavity and the role of RP105.

FIGURE 3

Splenic or peritoneal cavity B cells from F1(B6xBc) were cultivated under limiting dilution conditions in the presence of LPS, 30μg/mL, using S17 stroma cell line as feeder cells. The percentage of negative cultures is plotted against the B cell number/well, and frequencies of responding cells were calculated according to Poisson’s distribution. (A) FO and MZ B cells from BALB/cJ; (B) FO and MZ B cells from F1; and (C) B1a, B1b and B2 peritoneal cavity B cells from BALB/cJ. In (D), FO B cells from F1 mice were sorted according to the expression of RP105, as RP105low and RP105high, and then evaluated for their clonal response to LPS. Expression levels of RP105 in the different B cell subpopulations was measured by flow-cytometry. (E) Acomparison of RP105 expression in FO, MZ and B1 from BALB/cJ. (F) A comparison of RP105 expression in FO from BALB/c, C57BL/6J and F1 mice. (G) A comparison of RP105 expression in B1 from BALB/c, C57BL/6J and F1 mice. The shaded histograms indicate the background staining with control antibody isotype.