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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Hypertension. 2013 Feb 4;61(4):921–930. doi: 10.1161/HYPERTENSIONAHA.111.199588

Figure 2. Manipulating NEP levels affect migration and proliferation in PASMCs.

Figure 2

NEP−/− PASMCs were infected with lentivirus expressing full length human NEP. NEP+/+ PASMCs were infected with lentivirus expressing a peptidase dead mutant (NEPX) or transfected with NEP siRNA (10nMole/L). Migration and proliferation were assessed after 48h. Panel A shows the number of cells migrated/5 cm2 area from 3 different isolates along with a representative blot showing NEP levels after infection with lentivirus in null cells. Thymidine incorporation is shown in Panel B. The effect of expression of NEPX on migration is shown in Panel C along with a representative blot of NEPX levels. Panel D shows the effect on thymidine incorporation in NEP +/+ PASMCs. The effect of NEP knockdown with siRNA on migration along with a representative blot showing a decrease in NEP levels is shown in Panel E and Panel F shows effect on thymidine incorporation. *p≤ 0.05 for comparison of saline and treatment group. # p≤ 0.05 for comparisons between control and lentivirus or siRNA treatments.