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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Hypertension. 2013 Feb 4;61(4):921–930. doi: 10.1161/HYPERTENSIONAHA.111.199588

Figure 6. NEP substrates augment PDGF-induced migration and proliferation in NEP+/+ PASMCs.

Figure 6

NEP+/+ PASMCs were treated with ET-1(10nM) and FGF2 (10ng/ml) in the presence or absence of PDGF (10ng/ml) and effects on migration and proliferation were assessed. Panel A shows effects of ET-1 and FGF2 on PDGF induced migration in a scratch assay. Panel B shows effect of ETAR antagonist Ambrisentan (10μM) and PP2 (10μM) on PDGF+ET-1 induced migration. Panel C shows the effect of ET-1 and FGF2 on PDGF-induced thymidine incorporation. Panel D shows the effect of PP2 (10μM) and neutralizing FGF2 antibody (1μg/ml) on PDGF+FGF2 induced thymidine incorporation. *p≤ 0.05 for comparisons of control saline to PDGF (n=3). # p≤ 0.05 for comparisons of −PDGF treatment to +PDGF.