Skip to main content
. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: J Am Chem Soc. 2013 Apr 8;135(15):5895–5903. doi: 10.1021/ja401537t

Table 5.

Kinetic Parameters for the Inhibition of pNPA Hydrolysis by Zn(II)-bound TRI Peptides at pH 8.5a

Peptide
complexb& KI (M)		 [OAc−]
[M]		 kcat/KM
[M−1 s−1]		 kcat [s−1] KM [mM]
[Zn(II)(H2O/OH−)]N 0 3.2 ± 0.1 0.0065 ± 0.0006 2.0 ± 0.3
(TRIL2WL23H)3n+ 0.1 2.51 ± 0.05 0.0068 ± 0.0004 2.7 ± 0.2
   KI (M) = 0.34 ±
0.01 0.25 2.01 ± 0.07 0.0058 ± 0.0006 2.9 ± 0.4
0.435 1.32 ± 0.07 0.0067 ± 0.0021 5.1 ± 1.9
[Hg(II)]S 0 5.4 ± 0.1 0.011 ± 0.001 2.0 ± 0.2
[Zn(II)(H2O/OH−)]N 0.1 4.5 ± 0.2 0.012 ± 0.001 2.6 ± 0.4
(TRIL9CL23H)3n+
   KI (M) = 0.32 ± 0.2 3.6 ± 0.2 0.010 ± 0.002 2.6 ± 0.6
0.01 0.4 2.5 ± 0.1 0.012 ± 0.003 5.0 ± 1.2
0.6 1.8 ± 0.1 0.010 ± 0.003 5.7 ± 1.8
[Zn(II)(H2O/OH−)]N 0 2.54 ± 0.07 0.0039 ± 0.0002 1.5 ± 0.1
[Hg(II)]S 0.1 1.65 ± 0.02 0.0047 ± 0.0002 2.9 ± 0.2
(TRIL9HL23C)3n+ 0.25 1.09 ± 0.06 0.0036 ± 0.0008 3.3 ± 0.9
   KI (M) = 0.20 ±
0.01 0.5 0.72 ± 0.03 0.0042 ± 0.0012 5.8 ± 1.9
[Hg(II)]S 0 2.06 ± 0.06 0.0058 ± 0.0006 2.8 ± 0.4
[Zn(II)(H2O/OH−)]N 0.2 1.42 ± 0.04 0.0078 ± 0.0014 5.5 ± 1.1
(TRIL9CL19H)3n+ 0.4 0.94 ± 0.06 0.006 ± 0.003 6.5 ± 3.3
   KI (M) = 0.36 ±
0.01 0.6 0.73 ± 0.03 0.007 ± 0.003 9.1 ± 47
a

Error bars result from fitting all individual initial rates measured (three per concentration of substrate, without averaging) to the Michaelis Menten equation in Prism 5 (GraphPad Software).

b

20–50 µM active Zn(II)-bound peptide complex.