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Cdc42 biosensors based on the new dyes show the localization of Cdc42 activation in moving MEF cells. Cells were injected with CBD biosensor labeled with mero61 (A), mero62 (B), or mero87 (C). Biosensors show increased Cdc42 activation in areas where the cell edge is protruding, as indicated by white arrows (see supplemental movies S1 – S3). Some activity is also seen at perinuclear compartments and in the nucleus of some cells, consistent with previous observations.10
