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. 2013 Apr 3;33(14):6143–6153. doi: 10.1523/JNEUROSCI.5399-12.2013

Figure 4.

Figure 4.

p38α KO microglia fail to respond morphologically to a diffuse brain injury. A, IBA1 IHC shows a strong increase in staining and morphological alterations in the WT mFPI mice post-injury (p.i.), which were absent in the KO mFPI mice 6 h after injury and remained diminished in the KO mice 7 d after injury. Arrows indicate clusters of microglia around what appears to be a blood vessel. B, Quantification shows a significant increase in IBA1 staining in the injured WT mice at 6 h, which persists to 7 d after injury. Microglia in the KO mice demonstrated a failure to activate during the acute (6 h) or chronic phase (7 d) after the injury. C, CD68 IHC further supports the lack of a microglia morphological response to the diffuse brain injury in the absence of p38α, both at acute time points and during the chronic phase after the injury. D, Quantification shows a significant increase in CD68 staining in the WT-injured mice at 6 h that persisted to 7 d after injury, with minimal response in the KO mice. Data are presented as percentage of sham; n = 6–8 per group. *p < 0.05. **p < 0.01.