(A) MD or BD-deleted TnT3 COOH-terminus fused to DsRed. (B) C2C12 cells expressing MD or BD deletion constructs, which both localized exclusively in the nucleus, but enrichment in the nucleolar area was reduced, particularly for TnCT-BD/DsRed. Nuclei (blue, Hoechst 33342), TnT3-DsRed fusion protein (red). Scale bar, 50 µm.