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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2013 Feb 1;70(3):134–147. doi: 10.1002/cm.21095

Figure 6. KLKRQK is a genuine NLS and plays a major role in TnT3 nuclear localization.

Figure 6

(A) Schematic representation of DsRed-fused KLKRQK alone (NLS/DsRed) and full-length TnT3 devoid of this sequence (TnFL-ΔNLS/DsRed). (B) NLS/DsRed shows an exclusive nuclear localization with an enriched nucleolar area in C2C12 cells. (C) TnFL-ΔNLS/DsRed shows exclusion from the C2C12 nucleus, and only a minor fraction is localized in the nucleolar area (arrows). Nuclei (blue, Hoechst 33342), TnT3-DsRed fusion protein (red). Scale bar, 50 µm.