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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2013 Feb 1;70(3):134–147. doi: 10.1002/cm.21095

Figure 9. TnT3 cytotoxicity is gradually reduced by truncating the TnT3 COOH-terminus.

Figure 9

C2C12 cells transfected with various TnCT/DsRed constructs or DsRed alone for 48 hours were analyzed by flow cytometry. Data were collected on at least 50,000 freshly stained cells and at least 1000 DsRed positive cells and analyzed for staining with the cell death marker Annexin V (n=3). (A) Representative analyses of Annexin V staining after DsRed pregating. SSC, side scatter. (B) The percent of total apoptotic and necrotic cells (Annexin V+) was compared among different constructs. (C) A representative fluorescence image showing that C2C12 cells expressing TnCT-KL/DsRed or DsRed remain healthy in extended GM cultures and survive and fuse into myotubes in differentiation medium. Arrows indicate nuclear line up or elongation (D). (E) Brightfield merged with DsRed image showing that TnCT-KL/DsRed is localized in myotube nuclei, while DsRed alone is expressed throughout the myotube. Scale bars, 50 µm.