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. 2013 Jun 1;24(11):1661–1675. doi: 10.1091/mbc.E12-12-0908

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© 2013 Beaty et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — β1 integrin promotes invadopodial matrix degradation in 3D extracellular matrix. (A–D) 3D extracellular matrix invadopodium assay. (A) Representative maximum-intensity Z‑projection multiphoton images of MDA-MB-231 cells transiently transfected with control or β1 integrin siRNA and TagRFP-cortactin. Cells were embedded in a 3D ECM gel consisting of type I collagen, DQ-type I collagen, and Matrigel for 36 h. (B) Quantification of invadopodium number in control, β1 integrin–depleted, and mab13-treated cells in 3D matrix. n > 115 cells; three independent experiments. *p < 1.27E-15, compared with control siRNA. (C) Quantification of invadopodium length in 3D matrix. n > 115 cells; three independent experiments. *p < 0.013, compared with control siRNA. (D) Quantification of mean DQ-collagen (degradation) MFI per protrusion > 7 μm. n > 115 cells; three independent experiments. *p < 3.15E-9, compared with control siRNA.