FIGURE 7:
dSkp2 has a role in regulating Dap protein level in vivo. (A–E) Knockdown of dSkp2 leads to an increase in fluorescence signals for 4xMyc-Dap in wing discs. 4xMyc-dap51 was expressed in the posterior regions of the wing discs under the control of en-Gal4 either alone (A, A′) or with the dSkp2 knockdown (B, B′) or with a simultaneous knockdown of dSkp2 and Cks85A (C, C′). The confocal images shown were from side-by-side experiments and were captured under identical imaging settings. (D, E) Mean number of clusters of fluorescence signals (referred to as dots) per disc and mean aggregate intensity (in arbitrary units), respectively, of the detected dots. Error bars, SD calculated across different discs in a group. n = 20 discs for each genotype. (F–I) Coexpression of HA-dSkp2 leads to a reduction in fluorescence signals for 4xMyc-Dap in wing discs. 4xMyc-dap10–1 was expressed in the posterior region of the wing discs under the control of en-Gal4 either alone (F, F′) or with HA-dSkp2 together (G, G′). The confocal images shown were based on side-by-side experiments with images captured under identical settings. (H,I) Mean number of fluorescent dots detected per disc and mean aggregate intensity (in arbitrary units) of the detected dots, respectively. n = 13 for 4xMyc-dap10–1 discs and 19 for 4xMyc-dap10–1&HA-dSkp2 discs. **p < 0.01 (Student's t test). Scale bar, 75 μm.