Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jun 1;24(11):1713–1724. doi: 10.1091/mbc.E12-11-0792

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Mori and Toda. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — Alp41 interacts with Alp1^D only when in its GDP-bound form. (A–C) Immunoprecipitation. IPs were performed using a doubly tagged strain expressing the mutated form of Alp41 tagged with GFP and Alp1^D-3Pk. First lane, control strain expressing GFP-Alp41 only; second lane, Alp1^D-3Pk only; third lane, the doubly tagged strain. A 30-μg amount of whole-cell extract was loaded as a control. (A) Wild-type Alp41, (B) GTP-bound Alp41-Q70L, and (C) GDP-bound Alp41-T47A. (D) Genetic interactions between Tbc1, Alp41, and Alp1^D. Mutants of each gene were crossed and growth/lethality observed. SL, synthetic lethality.