FIGURE 1:

Clathrin is required for ATP7A trafficking. HeLa cells were treated with transfection reagent alone (mock), transfected with nonsilencing siRNA (-ve) or siRNA directed against cav1, cav2, flot1, or CLTC. (A) Immunoblot shows depletion of target proteins (indicated above the blot). A total of 10 μg of total protein was loaded per lane. Proteins are detected with the antibodies indicated on the left. (B) Cells were treated with BCS (a–e), CuCl₂ (f–j), or CuCl₂ followed by washout (k–o) at 37°C before fixation and IF staining with anti-ATP7A antibody (green) and DAPI nuclear stain (blue). Scale bar, 10 μm; where scale bar is not shown, scale is equivalent to that of n.