Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jun 1;24(11):1735–1748. doi: 10.1091/mbc.E12-08-0625

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Holloway et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — Depletion of clathrin causes ATP7A to accumulate at the cell surface. CLTC-deleted or control HeLa cells transfected with nonsilencing siRNA (-ve) were treated with BCS or CuCl₂. Cell surface proteins were labeled with biotin at 4°C, followed by cell lysis and isolation of biotinylated cell surface proteins on NeutrAvidin beads. (A) Western blot of prebead lysate (ly) or eluted cell surface e1 protein samples (cs) probed with an antibody to ATP7A or actin. (B) Quantification of cell surface ATP7A, calculated as a percentage of total ATP7A in the cell lysate. The graph is representative of three independent experiments.