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. 2013 Jun 1;24(11):1735–1748. doi: 10.1091/mbc.E12-08-0625

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© 2013 Holloway et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — ATP7A internalization is inhibited in cells expressing AP180-C. Transiently transected HeLa cells expressing myc-tagged AP180-C were subjected to treatment with BCS, CuCl₂, or CuCl₂, followed by washout. AP180-C is detected with an antibody against the myc epitope (a–c, g–i red), and cells are counterstained for ATP7A (d–f, g–i green). Scale bar, 10 μm.