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. 2013 Jun 1;24(11):1765–1775. doi: 10.1091/mbc.E12-12-0907

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© 2013 Nakatsukasa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Increased proteasome association with the Hrd1 core complex when Cdc48p is inactivated. (A, B) The indicated yeast strains were lysed in liquid nitrogen, and proteins were cross-linked with DSP. The membranes were collected and solubilized, and 3xFLAG-tagged Rpt5p was immunoprecipitated. Bound proteins were detected with specific antibodies after DTT treatment. The asterisk shows a nonspecific band that that cross-reacted with anti-FLAG antibody after the immunoprecipitation. (C) A model for the formation of the stalled retrotranslocation complex (see the text for details). As in Figure 5C, note that only a part of the ubiquitinated protein is drawn as a dotted line, because it may loosely associate with Hrd1p.