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. 2013 Jun 1;24(11):1776–1790. doi: 10.1091/mbc.E13-01-0026

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© 2013 Giridharan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Complex interactions between MICAL-L1, Synd2, and EHD1 in tubular localization and biogenesis. (A–D) Cells treated for 72 h with MICAL-L1 siRNA were transfected for the last 48 h with either siRNA-resistant, wild-type, HA-tagged MICAL-L1 or mutant constructs. The cells were then immunostained with anti-HA and anti-Synd2 antibodies. Bar, 10 μm. (E) HeLa cells treated as in A were quantified to assess the percentage of cells with Synd2 localized to tubular membranes. Error bars, SE. (F) Cells treated for 72 h with Synd2 siRNA were transfected for the last 48 h with siRNA-resistant, wild-type, HA-tagged Synd2. Cells were then immunostained with anti-HA and anti–MICAL-L1 antibodies. Bar, 10 μm. (G) HeLa cells treated as in F were quantified to assess the percentage of cells with MICAL-L1 localized to tubular membranes. Error bars, SE. (H) Model illustrating the interaction profile between MICAL-L1, Synd2, and EHD1.