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. 2013 Jun 1;24(11):1776–1790. doi: 10.1091/mbc.E13-01-0026

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© 2013 Giridharan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Phosphatidic acid–rich LMVs undergo tubulation in the presence of F-BAR domain of Synd2 and CC domain of MICAL-L1. In vitro tubulation assays were performed with (A) PE-containing liposomes only, (B) PA-containing liposomes only, (C) PE-containing liposomes and GST-Synd2, (D) PA-containing liposomes and GST-syndapin, (E) PE-containing liposomes and GST-Synd2-F-BAR, (F) PA-containing liposomes and GST-Synd2-F-BAR, (G) PE-containing liposomes and His-MICAL-L1-CC, or (H) PA-containing liposomes and His-MICAL-L1-CC. LMVs comprised a mass ratio of 70% PC, 10% NBD-PE, and 20% PE or PA. Insets depict the region in the white box. Bar, 10 μm.