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. 2013 May 30;7(5):e2161. doi: 10.1371/journal.pntd.0002161

Figure 1. Antigen detection capture ELISA for the identification of the proteins Li-isd1, Li-txn1, and Li-ntf2 in urine of VL patients and controls.

Figure 1

Urine specimens were from VL patients and normal, healthy control subjects. The following predetermined capture antibodies concentrations were used to coat the ELISA plates: antigen affinity-purified IgY anti-Li-isd1, 100 ng/well; antigen affinity-purified rabbit anti-Li-txn1 antibodies, 875 ng/well; and purified rabbit IgG anti-Li-ntf2 antibody (2,000 ng/well), or the combination of the three antibodies/well. Samples from VL patients (n = 20) were from Teresina, PI, Brazil. Control samples were from healthy individuals from countries where VL is endemic who were living in the United States. Dashed lines represent the cutoff values calculated as described in the text. These are representative results of at least three experiments performed at different times with the same urine samples and same capture ELISA.