Figure 2. Specificity of the capture ELISA assembled with a combination of antibodies specific to Li-isd1, Li-txn1, and Li-ntf2 antigens.

Urine specimens were from patients with cutaneous leishmaniasis (CL) (n = 10), Chagas disease (CD) (n = 8), schistosomiasis (SC) (n = 14), or tuberculosis (TB) (n = 10). Assay performed using capture ELISAs assembled all three antibodies in a single well as described in the legend for Figure 1. Dashed line represents the cutoff values, which was calculated using the average of the ODs obtained from the urine specimens from 20 normal, healthy control subjects plus 3 SDs.