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. 2013 May 30;9(5):e1003532. doi: 10.1371/journal.pgen.1003532

Figure 7. GLIS3 KD decreases SRp55 expression in INS-1E cells.

Figure 7

INS-1E cells were transfected with control, GLIS3 (A), SRp55 (B, C, D and E) and BimS siRNAs (E). After 48 h cells were incubated with cytokines (C–E) for measuring apoptosis and collected at different time points for real-time PCR analyses. (A) Representative blot of 2 independent experiments for SRp55 protein expression after GLIS3 KD (n = 7; densitometry is provided in Results); (B) SRp55 mRNA expression after KD with two different siRNAs (SRp55#1 and SRp55#2); (C) mRNA expression of BimS after SRp55 KD and exposure to cytokines; (D, E) apoptosis in cells transfected with SRp55 and/or a Bim siRNA targeting preferentially BimS (siBimS). Apoptosis was measured using nuclear dyes. Results are means ± SEM (n = 4–7). * P<0.05, ** P<0.01, *** P<0.001 vs. siCTL without cytokines @@@ P<0.001 vs. siSRp55#1; ### P<0.001 vs. siSRp55#2; &&& P<0.001 vs. siBimS; +++ P<0.001 vs. siSRp55 + siBimS; $ P<0.05 or $$ P<0.01 as indicated by the bars. Paired t- test (7B and 7C) or ANOVA followed by paired t-test with Bonferroni's correction (7D and 7E).