Figure 2. Validation of tracking function on simulated data.

The fraction of detected particles and fully reconstructed tracks (number of true positives/ground truth (GT)) as a function of the ratio of the average nearest neighbor distance over the average displacement a/d. Image stacks with 150 particles were simulated over 30 frames. In the first image frame particles were located at a distance 5×radius of Airy disk from each other and then moved assuming Brownian motion in subsequent frames. d was varied. Each data point shows the mean value of 50 repetitions.