Table 1. Extracted parameters, diffusion coefficients and fractions for all experimental conditions.

	# cells	# tra-jectories	l* [frames]	a [µm]	Dm * [µm2/s]	Dim * † †[µm2/s]	fm * [%]	d ‡ [nm]	a/d	β
Control	40	1465	22±3	1.7±0.4	0.14±0.02	0.015±0.007	73±4	136±10	13±3	5±2
Latrunculin	46	1622	21±3	1.7±0.3	0.14±0.01	0.013±0.003	70±3	136±50	13±3	5±2
MβCD	33	1287	25±3	1.4±0.5	0.07±0.02	0.011±0.003	69±4	96±14	15±5	4±2

l denotes the trajectory length,

^*Trajectories were randomly grouped in 10 subsets and their JD distributions fitted to equation 7. Errors are given by the standard deviation of the obtained D_m-, D_im- and f_m-values. ^†Note that the immobile population is static within the localization precision σ = 25±10 nm (Note S4 and Fig. S8 in File S1). SNR = 11 for all cell experiments and 14 for the experiments to determine the localization precision.

^‡ d = , with Δt = 33 ms for all experiments.