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. 2013 May 30;8(5):e64088. doi: 10.1371/journal.pone.0064088

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© 2013 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cells from two clones of normal keratinocytes (HNK1, HNK2) and two cancer cell lines (OECM1, SAS) were examined. (B) Clinical tissue samples of four grossly normal biopsies and five pieces of cancer tissues from head-neck cancer patients were examined. Cellular or nuclear proteins were extracted and subjected to immunoblot analysis for the expression of DSG3, plakoglobin, c-myc, Cyclin D1 and MMP7, as described in the method section. Expressions of Actin served as internal control for cellular protein, and HDAC for nuclear protein. Cellular protein expression was normalized by Actin and nuclear protein by HDAC after determination of each band density by Image J software.