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. 2013 May 30;8(5):e64781. doi: 10.1371/journal.pone.0064781

Figure 4. Disruption of the interaction between HPV16 E7 and pRb by aptamer A2 in vitro.

Figure 4

(A) GST pull-down assay in the presence and absence of aptamer A2. 1 µg of GST-E7 (54 µM) bound to GM-beads was incubated with 300 µg of protein from a HaCaT cell lysate (as a source of pRb) for 1 hour at 4°C in the presence of 0, 5, 10 or 15 µg of A2. Bead-bound GST alone was incubated with either 0 or 15 µg (11 µM) A2. GST proteins and interacting proteins were isolated from the reaction and analysed by immunoblot using antibodies to pRb and GST. (B) Densitometric analysis of the interaction between GST-E7 and pRb in the absence and presence of A2, standard errors relating to three separate experiments are shown. *indicates p<0.05, **indicates p<0.01.