Figure 1. Effect of cell contraction inhibitors on selected known ROCK substrates (A) and “classical” integrin signals (B) after attachment of GD25β1 (n = 2), GD25 (n = 3) and BJ hTERT (n = 3) cells.

The cells were treated with 100 µM of the non-muscle myosin inhibitor Blebbistatin, Blebbistatin vehicle control DMSO (DMSO (BL)), or 10 µM of the ROCK inhibitor Y27632 as indicated, and seeded on non-adhesive PL or FN (GD25) or FN in the presence of soluble cyclic RGD peptide (GD25β1 and BJ hTERT). After attachment for the indicated times, the cells were solubilized and subjected to western blot analysis. (C) TIRF recording of cell spreading kinetics on the β1 integrin-selective ligand invasin for GD25β1 control cells (black, n = 24) and GD25β1 cells treated with 10 µM ROCK inhibitor Y27632 (red, n = 17).