Table 1. Comparison of genomic DNA and whole genome amplified DNA from DBS.

		Portion of DBS	DBS per genotype run	Samples expected to reach 10 ng/µl	Time1	Cost per sample	SNP error rate2	SNP failure rate3	Useful for CNV analysis	Useful for DNA sequencing
gDNA	Initial extraction	17%	4%	37%	22 h	$1	1.6×10−5	0.028	Yes	Yes
	+1 iteration	33%	8%	72%
	+2 iterations	50%	13%	93%
	+3 iterations	67%	17%	99%	22 h	$1
wgaDNA	DNA extraction and amplification	7%	2%	100%	24 h	$5	1.4×10−4	0.034	No	?

¹Time for two 96 well extraction plates, not including time to catalog samples; All iterations of samples from the original two 96 well plates typically fill two new 96 well plates (22 hours includes all iterations).

²Mean SNP genotyping replication error rate from Figure 1B.

³Mean SNP genotyping failure rate (protocol development sample set); gDNA and wgaDNA failure rates are significantly different (p = 2.6×10⁻⁷, Student’s t-test).