Table 1. Strains, plasmids and primers used.
| Strains | Relevant phenotype or genotype | Reference or Source |
| B. breve UCC2003 | Infant isolate | [37] |
| B. breve NCFB2258 | Infant isolate | NCFB |
| E. coli EC101 | Cloning host, repA+, Kanr | [59] |
| Plasmids | ||
| pMOD2 | Source of tn5 terminal ends, AmpR | Epicentre Biotechnology |
| pMOD2-TetW | TetR derivative of pMOD2 | This study |
| Primers | Sequence | |
| TetW-Fw | 5′-CGCTAGTCTAGAGCTCATGTACGGTAAGGAAG-3′ | This study |
| TetW-Rev | 5′CGCTAGGCATGC AAAACCCTCGGTCGGTCTGACCGGGGGTTTTGATTACATTACCTTCTGAAACATATGGC-3′ | This study |
| pMOD<MCS>Fw | 5′-ATTCAGGCTGCGCAACTGT-3′ | Epicentre Biotechnology |
| pMOD<MCS>Rev | 5′-GTCAGTGAGCGAGGAAGCGGAAG-3′ | Epicentre Biotechnology |
| i-PCR-Fw | 5′-GCATACCGTACTGATCTG-3′ | This study |
| i-PCR-Rev | 5′-CAATCATACCGGCTTCC-3′ | This study |
| pMOD-fw-seq | 5′-GCCAACGACTACGCACTAGCC-3′ | Epicentre Biotechnology |
| pMOD-rev-seq | 5′-GAGCCAATATGCGAGAACACC-3′ | Epicentre Biotechnology |
Restriction sites are underlined and a rho-transcriptional terminator sequence is highlighted in bold. NCFB, National Collection of Food Bacteria.