Figure 1. OCT4-specific IP-10 production in peripheral blood mononuclear cells from healthy subjects. (A) Freshly isolated peripheral blood mononuclear cells (PBMCs) were labeled for intracellular interferon γ (IFNγ)-inducible protein 10 (IP-10). (B and C) Alternatively, PBMCs were stimulated with an immunoreactive OCT4-derived peptide for 48 h, followed by the assessment of intracellular IP-10 by flow cytometry (B) or IP-10 secretion by ELISA (C). Non-stimulated PBMCs were included as a negative control. No brefeldin A was added to the culture, and a minimum of 1×10⁵ cells was analyzed.