Table 1.
Instances in Which All Three Donors Had >1.8-Fold Differences in MC Activity with or without PCM Priming
| MC activity | Culture time (hours) | Biomaterial surface | PCM priming (+/−) | Donor 1 | Donor 2 | Donor 3 |
|---|---|---|---|---|---|---|
| Viable adherent MCs/mm2 | 170 | PEG hydrogel | − | 207 | 241 | 82 |
| 170 | PEG hydrogel∗ | + | 888 | 1058 | 235 | |
| Fold-change with PCM priming | 4.3 | 4.4 | 2.9 | |||
| 170 | PDMS | − | 116 | 90 | 16 | |
| 170 | PDMS | + | 222 | 217 | 80 | |
| Fold-change with PCM priming | 1.9 | 2.4 | 4.9 | |||
| Total (viable and necrotic) adherent MCs/mm2 | 170 | PEG hydrogel | − | 226 | 272 | 103 |
| 170 | PEG hydrogel∗ | + | 950 | 1111 | 270 | |
| Fold-change with PCM priming | 4.2 | 4.1 | 2.6 | |||
| IL-1β (pg/mL)† | 98 | PDMS | − | 20 | 18 | 18 |
| 98 | PDMS | + | 51 | 60 | 56 | |
| Fold-change with PCM priming | 2.5 | 3.3 | 3.1 | |||
| TNF-α (pg/mL) | 98 | PDMS | − | 62 | 37 | 9 |
| 98 | PDMS | + | 173 | 218 | 17 | |
| Fold-change with PCM priming | 2.8 | 5.9 | 1.8 | |||
∗
P = 0.01 to P = 0.05, significant difference in the mean of the three donors with PCM priming based on one-way analysis of variance combined with Bonferroni's multiple comparison post tests.
†
There were also >1.8-fold increases in IL-1β expression with PCM priming in MC cultures on PEG hydrogels and PDMS at 170 hours, but because some of the IL-1β values were extrapolated below the standard curve at 170 hours, these increases in IL-1β expression with PCM priming were not included in the table.