Table 2.

Instances in Which All Three Donors Had >1.8-Fold Differences in Cell Activity between the PMN-MC Direct Co-Culture and the Sum of PMN and MC Monocultures

Cell activity	Culture time (hours)	Biomaterial surface	Direct co-culture (PMN-MC) or sum of PMN and MC monocultures (PMN + MC)	Donor 1	Donor 2	Donor 3
Viable adherent cells/mm2	48	PDMS	PMN + MC	412	48	185
	48	PDMS	PMN-MC	833	116	340
	Fold-change with direct co-culture (PMN-MC)			2.0	2.4	1.8
	48	TCPS	PMN + MC	187	88	66
	48	TCPS	PMN-MC	349	173	270
	Fold-change with direct co-culture (PMN-MC)			1.9	2.0	4.1
	168	PEG hydrogel	PMN + MC	44	46	41
	168	PEG hydrogel	PMN-MC	22	3	22
	Fold-change with direct co-culture (PMN-MC)			0.50	0.06	0.53
IL-6 (pg/mL)∗	48	TCPS	PMN + MC	14	797	146
	48	TCPS	PMN-MC	7	44	78
	Fold-change with direct co-culture (PMN-MC)			0.47	0.06	0.54
IL-8 (pg/mL)	168	TCPS	PMN + MC	795	897	353
	168	TCPS	PMN-MC	1436	2253	919
	Fold-change with direct co-culture (PMN-MC)			1.8	2.5	2.6

^∗There were also >1.8-fold decreases in IL-6 expression between the PMN-MC direct co-culture and the sum of the PMN and MC monocultures on TCPS at 96 hours, but because some of the IL-6 values were extrapolated below the standard curve at 96 hours, these decreases in IL-6 expression were not included in this table.