Figure 1.

Conditional Pten and Tp53 heterozygous deletion in the SM lineage leads to HGUPS, LMS, and CS. A: Survival plot for mice with the indicated genotypes as a function of days. A statistically significant decrease in lifespan was found for Pten^Δ/+Tp53^Δ/+ mice compared with Pten^Δ/+Tp53^+/+ mice (^∗∗P = 0.0024) and Pten^+/+Tp53^Δ/+ mice (^∗∗∗P < 0.0001 ). B: Macroscopic images of a uterine LMS in a Pten^+/+Tp53^Δ/+ mouse (top panel) and an abdominal HGUPS in a Pten^Δ/+Tp53^Δ/+ mouse (bottom panel). C: H&E and IHC stainings of sections of the three sarcoma subtypes found on Pten^Δ/+Tp53^Δ/+ mice with antibodies against desmin and PCNA. The percentage of PCNA-positive cells is indicated. Scale bars, ×20 (250 μm); ×40 (125 μm). D: H&E and IHC staining for desmin in metastases found in the indicated organs. The top two rows correspond to a Pten^+/+Tp53^Δ/+ LMS, and the bottom panel depicts a Pten^Δ/+Tp53^Δ/+ CS. E: Top panel, scheme of primers used for Pten and Tp53 PCR-based assays to genotype or sequence for mutations and/or deletions. ORF, open reading frame. Bottom panel, electrophoresis of PCR products from genomic DNA isolated from tumor tissue using the indicated primer pairs. Floxed denotes the recombined allele after cre-mediated recombination. F: Gel electrophoresis of PCR amplification products for Tp53 (top two lanes) and Pten (bottom five lanes) in DNA from short-term cultures of the corresponding murine tumors. In parenthesis, primer pairs used for detection. GAPDH is used as a loading control.