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. 2013 Jun;182(6):2015–2027. doi: 10.1016/j.ajpath.2013.02.035

Figure 2.

Figure 2

Pten silencing in Tp53 heterozygous mesenchymal stem cells and tumor cells exacerbates their oncogenic behavior. Murine BM-MSCs were infected with Ad5-CMV-Cre-GFP and subjected to colony formation (A) and proliferation assays (B). Relative proliferation at day 6 after infection is shown. C: Western blot analysis of two murine Pten+/+Tp53Δ/+ HGUPS cell lines infected with shPten (sh) or nonsilencing control (V) and parental (C) cells. Colony formation assay (quantitated in the adjacent histogram) (D) and growth curve (E) of shPten-infected HGPUS cells compared with nonsilencing control. F: Representative images of a transwell invasion assay on two HGUPS cell lines transduced with nonsilencing control or shPten lentivirus. Graphs indicate relative number of cells per field. B and D–F: Representative of three independent experiments ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.