Figure 3.

The Notch signaling pathway is up-regulated in Pten^Δ/+Tp53^Δ/+ murine HGUPS. A: Histogram of pathways up-regulated in Pten^Δ/+Tp53^Δ/+ tumors according to DAVID.^27,28B: GSEA performed on genes differentially expressed (fold change of >1.25 and P < 0.05) in Pten^Δ/+Tp53^Δ/+ compared with Pten^+/+Tp53^Δ/+. C: Selected differentially expressed genes found in relevant pathways. D: Validation of the RNA array data by quantitative real-time RT-PCR comparing Pten^Δ/+Tp53^Δ/+ and Pten^+/+Tp53^Δ/+ murine HGUPS cell lines. The fold change (FC) is shown for different genes. GAPDH was used for normalization. Error bars represent SD between two experimental replicates. ^∗P < 0.05, ^∗∗P < 0.01.