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. 2013 Jun;182(6):2015–2027. doi: 10.1016/j.ajpath.2013.02.035

Figure 4.

Figure 4

Increased Notch signaling partially accounts for the higher invasive and clonogenic potential of PtenΔ/+Tp53Δ/+ sarcoma. A: Relative expression of Hes1 by quantitative real-time RT-PCR of PtenΔ/+Tp53Δ/+ and Pten+/+Tp53Δ/+ HGUPS murine sarcoma cell lines, with (+) or without (−) 500 nmol/L Compound E. Relative values referred to R18S1, used as a housekeeping gene. B, Left panel: Transwell invasion assay for PtenΔ/+Tp53Δ/+ and Pten+/+Tp53Δ/+ HGUPS sarcoma cell lines, with (+) or without (−) 500 nmol/L Compound E. Data are given as means ± SD. Right panel: Representative images of the invasion assay. C: Relative expression of Hes1 in Pten+/+Tp53Δ/+ HGUPS transduced with shPten or vector (v), in the presence (+) or absence (−) of 500 nmol/L Compound E. Clonability (D) and transwell invasion assay (E) in Pten+/+Tp53Δ/+ HGUPS transduced with shPten or vector (v), with (+) or without (−) 500 nmol/L Compound E. Data are given as means ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.