Table 2.

Cell Number of Intratumoral Immune Subsets per 100 mg of Tumor (×10⁶)

	Tumor Cells	Total CD45+	CD8+ T cells	Total CD4+ T cells	Tregs	NK cells	Macrophages	Monocytes	Dendritic cells
Day 7	13 ± 2	11 ± 1	0.3 ± 0.04	0.2 ± 0.03	0.1 ± 0.01	0.6 ± 0.08	4.8 ± 0.7	2.5 ± 0.6	0.4 ± 0.06
Day 9 UT	14 ± 2	11 ± 2	0.7 ± 0.2	0.4 ± 0.09	0.3 ± 0.03	0.4 ± 0.08	5.7 ± 1	2.9 ± 0.8	0.4 ± 0.08
Day 9 IR	8.7 ± 1	9 ± 1	0.3 ± 0.05	0.1 ± 0.02∗	0.07 ± 0.004∗	0.3 ± 0.06	4.4 ± 0.4	2.5 ± 0.7	0.3 ± 0.05
Day 11 UT	16 ± 1	13 ± 2	2.4 ± 0.6	0.4 ± 0.06	0.3 ± 0.04	0.4 ± 0.07	6.7 ± 0.9	2.2 ± 0.4	0.2 ± 0.04
Day 11 IR	4.7 ± 0.8∗	18 ± 4	2.7 ± 1	0.3 ± 0.06	0.1 ± 0.04∗	0.6 ± 0.1	6.8 ± 1	4.3 ± 0.9	0.4 ± 0.1

Cell populations are defined as follows: tumor cells: CD45⁻, forward scatter^hi; CD8⁺ T cells: CD45⁺, CD8⁺; CD4⁺ T cells: CD45⁺, CD4⁺; Tregs: CD45⁺, CD4⁺, FoxP3⁺; NK cells: CD45⁺, NK1.1⁺; macrophages: CD45⁺, F480^hi, forward scatter^hi; monocytes: CD45⁺, F480^intermediate, forward scatter^low; dendritic cells: CD45⁺, F480^-, CD11b⁺, CD11c⁺. Data were generated from at least six individual tumors per group to determine the means ± SEM for each subset except in Tregs, where three individual tumors were used. Comparisons were made between the UT and IR values at either the day 9 or day 11 time points in each group (but not across groups).

IR, irradiated; UT, unirradiated.

^∗P < 0.05 as determined by one-way analysis of variance and the Bonferroni post hoc test.