Table 3.
Washed Platelet Aggregation
| Variable | Washed platelets |
|||||
|---|---|---|---|---|---|---|
| ADP (10 μmol/L; n = 3)∗ |
Col (2 μg/mL; n = 5) |
AA (0.5 mmol/L; n = 5) |
||||
| WT | Fut−/− | WT | Fut−/− | WT | Fut−/− | |
| LT (seconds) | 14.0 ± 2.1 | 14.0 ± 2.5 | 95.2 ± 7.7 | 110 ± 11.3 | 35.0 ± 0.8 | 36.6 ± 1.1 |
| Slope | 67.7 ± 3.4 | 70.0 ± 2.5 | 59.0 ± 1.8 | 58.7 ± 1.5 | 71.1 ± 5.6 | 67.4 ± 1.5 |
| MA (%) | 77.7 ± 0.7 | 82.3 ± 3.3 | 81.8 ± 1.2 | 84.4 ± 0.5 | 73.5 ± 6.2 | 74.8 ± 1.3 |
All values represent means ± SD.
AA, arachidonic acid; Col, collagen; LT, lag time; MA, maximum amplitude.
Human fibrinogen (15 μg/mL final concentration) was added for ADP-stimulated washed platelet aggregation.