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. 2013 Jun;182(6):2241–2253. doi: 10.1016/j.ajpath.2013.02.011

Figure 2.

Figure 2

Expression of Col8a1 mRNA is suppressed by ApoE and HDL. A: mRNA levels for the α1 chain of type VIII collagen (Col8a1) were significantly increased in the aortic arch (Arch) and thoracic aorta (TA) of ApoE−/− mice (hatched bars) compared with wild-type C57BL/6 mice (black bars). mRNA levels for the α2 chain of type VIII collagen (Col8a2) were low and did not differ between genotypes. All values are expressed as a fold change compared with levels of mRNA for Col8a1 in wild-type aortic arch. ∗P ≤ 0.05 compared with corresponding wild-type aorta; n = 4 samples of aortic segments pooled from 3 to 4 mice each. B: Primary mouse SMCs were incubated in 10% FBS in the absence or presence of ApoE3, ApoA1, HDL, or LDL. mRNA expression for Col8a1 was measured using RT-qPCR and is expressed as a percentage of FBS control. ∗P ≤ 0.05 compared with FBS control; n = 4 experiments.