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. 2013 Jun;182(6):2241–2253. doi: 10.1016/j.ajpath.2013.02.011

Figure 4.

Figure 4

SMC accumulation and proliferation are reduced in Col8−/−;ApoE−/− mice. Representative longitudinal sections of the lesser curvature of the aortic arch after 12 weeks of an atherogenic diet and stained with antibody against α-smooth muscle actin (A) or antibody against Mac-2 to label macrophages (B), along with their respective negative controls. C: Percentage of plaque area stained positive for SMA at 6 weeks in Col8+/+;ApoE−/− (n = 11) and Col8−/−;ApoE−/− (n = 10) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 9) and Col8−/−;ApoE−/− (n = 8) mice. ∗P ≤ 0.05 comparing genotypes at the same time point. D: Percentage of plaque area stained positive for Mac-2 at 6 weeks in Col8+/+;ApoE−/− (n = 13) and Col8−/−;ApoE−/− (n = 13) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 7) and Col8−/−;ApoE−/− (n = 8) mice. E: Percentage of proliferating cells at 6 weeks in Col8+/+;ApoE−/− (n = 8) and Col8−/−;ApoE−/− (n = 11) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 13) and Col8−/−;ApoE−/− (n = 7) mice. ∗P ≤ 0.05 comparing genotypes at the same time point. F: Percentage of apoptotic cells at 6 weeks in Col8+/+;ApoE−/− (n = 7) and Col8−/−;ApoE−/− (n = 12) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 9) and Col8−/−;ApoE−/− (n = 8) mice. Black bars represent values from Col8+/+;ApoE−/− mice, and hatched bars represent values from Col8−/−;ApoE−/− mice. Values are given as means ± SEM. Scale bars: 100μm.