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. 2013 Jun;182(6):2241–2253. doi: 10.1016/j.ajpath.2013.02.011

Figure 5.

Figure 5

Matrix accumulation and fibrous cap thickness are decreased, and plaque necrotic core size is increased in plaques from Col8−/−;ApoE−/− mice. Representative longitudinal sections of plaques from the lesser curvature of the aortic arch after 12 weeks of an atherogenic diet and stained with PSR to label collagen (A) or Verhoeff–van Gieson to label elastin (B). C: Percentage of plaque area stained positive with PSR at 6 weeks in Col8+/+;ApoE−/− (n = 12) and Col8−/−;ApoE−/− (n = 13) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 9) and Col8−/−;ApoE−/− (n = 8) mice. D: Fibrillar collagen content, measured as birefringence via PolScope at 6 weeks in Col8+/+;ApoE−/− (n = 12) and Col8−/−;ApoE−/− (n = 13) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 9) and Col8−/−;ApoE−/− (n = 8) mice. E: Percentage of elastin-positive plaque area at 6 weeks in Col8+/+;ApoE−/− (n = 7) and Col8−/−;ApoE−/− (n = 15) mice or at 12 weeks in Col8+/+;ApoE−/− (n = 8) and Col8−/−;ApoE−/− (n = 7) mice. F: Relative fibrous cap thickness in Col8+/+;ApoE−/− (n = 11) and Col8−/−;ApoE−/− (n = 8) mice at 12 weeks. G: Percentage of plaque occupied by necrotic core in Col8+/+;ApoE−/− (n = 12) and Col8−/−;ApoE−/− (n = 10) mice at 12 weeks. Black bars represent values from Col8+/+;ApoE−/− mice, and hatched bars represent values from Col8−/−;ApoE−/− mice. Values are given as means ± SEM. C–G: ∗P ≤ 0.05 comparing genotypes at the same time point. H: Classification of lesions found at 12 weeks in Col8+/+;ApoE−/− (n = 15) and Col8−/−;ApoE−/− (n = 16) mice. Scale bars: 100 μm.