Figure 1.

The N-terminal region of JAZ10.4 has a cryptic MYC2-interacting domain. A, Y2H assays of JAZ10.4 deletion constructs (DNA-binding domain bait fusions) with MYC2 and JAZ10.4 (activation domain prey fusions). Yeast strains coexpressing the indicated bait and prey proteins were plated on medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. Blue-color formation in streaked yeast cells is indicative of protein-protein interaction. Photographic images were taken after 30 h of incubation at 30°C. JAZ10.1 is included as a positive control. The ZIM (Z) and Jas domains are indicated. The solid black line denotes the C-terminal region that is specific for the JAZ10.4 isoform. B, C-terminal deletion constructs of JAZ10.4 were tested for interaction with MYC2 as described in A. C, Sequence similarity between the conserved Jas motif of JAZ10 and JAZ9 and the CMID near the N terminus of JAZ10. The underlined sequence shows the region of conservation, which includes a tribasic motif (boldface).