Figure 3.
The RRR motif is required for the JAZ10.4-mediated repression of JA responses. A, The photograph shows silique development in the wild type (WT) and transgenic lines that overexpress JAZ10.4 or JAZ10.4RRR→AAA. B, The photograph shows seedlings of the indicated genotypes grown for 10 d on Murashige and Skoog medium containing 20 μm MeJA. C, Quantification of JA-induced root growth inhibition in the wild type, 35S:JAZ10.4 (10.4), and eight independent JAZ10.4RRR→AAA lines. Seedlings were grown for 10 d on Murashige and Skoog agar plates containing or not containing 20 µm MeJA. The root length ratio was calculated by dividing the average root length of MeJA-treated seedlings by the average root length of seedlings of the same genotype grown in the absence of MeJA. Data show means ± se (n = 12 seedlings per genotype). The asterisk denotes a significant difference (P < 0.05, Student’s t test) in comparison with the wild type. [See online article for color version of this figure.]