Figure 4.

The ZIM domain of JAZ10 interacts with NINJA and is required for the repression of JA responses. A, Y2H assays depicting the interaction of JAZ10 or JAZ8 chimeric proteins (DNA binding-domain fusions [DB]) with MYC2, JAZ1, NINJA (NJA), and TPL (activation domain fusions [AD]). Empty vectors were included as negative controls. Sequence regions derived from JAZ10 and JAZ8 are shown in white and gray, respectively, together with various protein domains. E, EAR motif. Y2H assays were performed as described in Figure 1, except that the photographic image was taken after 48 h of incubation of yeast cells at 30°C. B, Overexpression of a JAZ8ΔEAR-ZIM10 chimeric protein confers insensitivity to JA in root growth assays. Seedlings were grown for 8 d on Murashige and Skoog agar plates containing or not containing 20 µm MeJA, and root length ratios were calculated as described in Figure 3C. Data show means ± se of at least 11 seedlings per genotype. Asterisks denote significant differences (P < 0.05, Student’s t test) in comparison with the wild type (WT).