Figure 6.

JA-induced expression of JAZ10.4 complements the JA-hypersensitive phenotype of jaz10-1. A, Root growth inhibition assay of the wild type (WT), jaz10-1 (jaz10), and five independent lines in which the pJAZ10:HA-JAZ10.4 transgene was introduced into the jaz10-1 mutant background. Root length was measured in 8-d-old seedlings grown on Murashige and Skoog medium containing 0, 10, or 20 µm MeJA. The root length ratio was calculated by dividing the average root length of seedlings grown in the presence of either 10 µm (gray bars) or 20 µm (black bars) MeJA by the average root length of seedlings of the same genotype grown in the absence of MeJA. Data show means ± se of 12 to 14 seedlings per genotype. Asterisks indicate significant differences in root length (P < 0.05, Student’s t test) in comparison between wild-type and jaz10 seedlings grown at the same concentration of MeJA or between wild-type and pJAZ10:HA-JAZ10.4 seedlings grown at the same concentration of MeJA. B, Accumulation of HA-JAZ10.4 protein in response to JA treatment. Ten-day-old liquid-grown seedlings of the indicated pJAZ10:HA-JAZ10.4 line were treated with 50 µm MeJA and harvested at various times (h) thereafter. As controls, seedlings were harvested immediately prior to treatment (0) and 8 h after mock treatment (C). Total protein was subjected to immunoblot analysis with an anti-HA antibody to detect HA-JAZ10.4. A Coomassie blue-stained gel of protein extracts was used as a loading control (CB). [See online article for color version of this figure.]